Traditionally, macroscopic and microscopic methods have been used as routine approaches for the identification Chinese Materia Medica (CMM). In the presence of morphological and tissue characteristics of CMM, they can be examined and identified unequivocally. In recent years, there has been a growing interest in the development of DNA-based methods as a complementary approach for species identification, especially for discrimination of closely related species. DNA-based testing methods and technical guidance have been published by international authorities such as Pharmacopoeia of the People’s Republic of China, U.S. Pharmacopeia and British Pharmacopoeia.
This research project aims at developing DNA barcode test method and establishing Reference DNA Sequence Library for Chinese Materia Medica (CMMRSL). Through the use of DNA technologies, we may enhance the genetic traceability between Chinese medicines and their plant / animal species origin for the sake of ensuring efficacy and safety of clinical application of Chinese medicines being used originated from proper medicinal plant/ animal. The method was validated in accordance with relevant HOKLAS requirements as well as developed a quality control plan for testing. The continuous development of CMMRSL currently curated reference DNA sequences of 30 commonly used CMM, including Panax ginseng C. A. Mey, Panax quinquefolium L., Panax notoginseng(Burk.) F. H. Chen, Cervus nippon Temminck and Cervus elaphus Linnaeus.
Our research outcomes is open to the public, especially to the CM and testing industries, not only to elevate local testing standards to provide versatile testing services for more economic opportunities but also promote the development of Chinese medicine testing and certification.